An Interview with David Drubin in Biowire

The May 2012 edition of Biowire, a publication of Sigma-Aldrich, includes an interview with David Drubin about the projects in our lab looking at clathrin-mediated endocytosis (CME) in mammalian cells using zing finger nuclease (ZFN) technology to undertake targeted genome modification. Traditionally, CME has been studied in cells in which fluorescently-tagged components of endocytic machinery are overexpressed using exogenous constructs. Data obtained in many labs using these methods suggested that CME was highly variable. Using ZFN technology, in collaboration with Sangamo Biosciences, our lab recently showed that CME is robust and efficient in mammalian cells.  The new results highlight the technical advantages of tagging genes at their endogenous loci, an approach that has been historically limited to genetically tractable organisms, such as the Drubin/Barnes Lab favorite Saccharomyces cerevisiae (budding yeast).  Emerging technologies, such as ZFNs and TALENs, however, are now making this sort of precise genomic manipulation possible in animal cells, including human cells, giving us new and powerful ways of studying cellular biology.

Cellular processes should be studied as close to their natural states as possible. I suspect that, as we see more uses of zinc finger nucleases [for tagging endogenous genes], people will find that they have been inadvertently perturbing the processes that they have been studying.

David Drubin (Biowire, May 2012)

Journal Club on Monday, April 30

For our next Journal Club, Rebecca Lu will present the following paper:

Membrane Fission Is Promoted by Insertion of Amphipathic Helices and Is Restricted by Crescent BAR Domains. Boucrot E, Pick A, Camdere G, Liska N, Evergren E, McMahon HT, Kozlov MM.  Cell. 2012 Mar 30;149(1):124-36. PMID: 22464325.

Lillie joined the Drubin/Barnes Lab!

The Drubin/Barnes Yeast and HeLa Cell Manufacturing Company is excited to welcome Lillie Cohn as a new graduate student in the lab!  Lillie is a first year in the Molecular and Cell Biology program at UC-Berkeley.  Her proposed thesis project is “The Meaning of Life.”  On this occasion, let us pause to consider these wise words of the sage Philosoraptor:

Journal Club on Monday, April 16

For our next Journal Club, Christa Cortesio will present the following paper:

Distinct and separable activities of the endocytic clathrin-coat components Fcho1/2 and AP-2 in developmental patterning.  Umasankar PK, Sanker S, Thieman JR, Chakraborty S, Wendland B, Tsang M, Traub LM.  Nat Cell Biol. 2012 Apr 8. doi: 10.1038/ncb2473. PMID: 22484487.

Journal Club on Monday, April 2

For our next Journal Club, Sun Hae Hong will present the following paper:

Influence of synaptic vesicle position on release probability and exocytotic fusion mode.  Park H, Li Y, Tsien RW.  Science.  2012 Mar 16;335(6074):1362-6.  PMID: 22345401.

Journal Club on Wednesday, February 20

For our next Journal Club, Akemi Kunibe will be presenting the following paper:

Membrane Tension Maintains Cell Polarity by Confining Signals to the Leading Edge during Neutrophil Migration. Houk AR, Jilkine A, Mejean CO, Boltyanskiy R, Dufresne ER, Angenent SB, Altschuler SJ, Wu LF, Weiner OD. Cell. 2012 Jan 20;148(1-2):175-88. PMID: 22265410

Journal Club on Monday, February 6

For our next Journal Club, Jasper Weinberg will be presenting the following paper:

A molecular switch on an arrestin-like protein relays glucose signaling to transporter endocytosis.  Becuwe M, Vieira N, Lara D, Gomes-Rezende J, Soares-Cunha C, Casal M, Haguenauer-Tsapis R, Vincent O, Paiva S, Léon S.  J Cell Biol. 2012 Jan 23;196(2):247-59.  PMID: 22249293